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KMID : 1094720130180020399
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 2 p.399 ~ p.405
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Improvement of welan gum production and redistribution of metabolic flux under pH control process in Alcaligenes sp. CGMCC2428
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Li Hui
Li Sha
Xu Hong
Chen Xiaoye
Ouyang Pingkai
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Abstract
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Batch fermentations of welan gum from Alcaligenes sp. CGMCC2428 at pH values of 5.5 ∼ 7.0 were studied. Based on the kinetic analysis, a pH control process for improving welan production was developed. By maintaining the culture pH at 7.0, the process significantly improved the maximal welan concentration and productivity to reach 25.1 ± 0.65 g/L and 0.42 ± 0.003 g/L/h, respectively, compared with those in native pH processes where pH value would decrease from 7.0 to 5.1 (18.5 ± 0.72 g/L and 0.28 ± 0.002 g/L/h). This improvement may be due to the increased metabolic flux of glucose-1-phosphate to welan induced by pH control process. Furthermore, scale-up fermentation under controlled pH was implemented using 300-L fermentor. The highest welan concentration of 27.5 ± 0.97 g/L was obtained. This simplified process proved effective in industry fermentation for high welan production.
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KEYWORD
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Alcaligenes sp. CGMCC2428, fermentation, welan, pH control process, metabolic flux analysis
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